Thanks, Zeke. That did it. I moved that file to a network drive (it was so fragmented it took over 70 minutes just to copy it!), and ran the defrag again. It all went off without a hitch and now I'm seeing glorious blue I'll have to ask the user sometime if she notices any performance improvements.

Now I've just got to do this on another machine that's been horribly fragmented. It's like 85% fragmented, has 9% of the disc free, and only 1% free for defragging. It's gonna be interesting.

Thanks guys for your help!