I've seen similar stuff happen - often with the "marked" flag. It was annoying for a while with no UI to change the flag on a playlist (JEmplode now supports this, but didn't at the time). I had to change my stored search to select only marked tunes.
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Toby Speight
030103016 (80GB Mk2a, blue)
030102806 (0GB Mk2a, blue)