hmmm - just run a test and downloaded two files I already have ripped myself, and they sound pretty much the same, both in the car and on my home setup....
And my friends typically reckon I am fussier about music quality than most.
YMMV
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Rory MkIIa, blue lit buttons, memory upgrade, 1Tb in Subaru Forester STi MkII, 240Gb in Mark Lord dock MkII, 80Gb SSD in dock