Well, since I've been travelling between Williamsburg and northern Virginia for over four years now, I've noticed regional differences between even these seemingly proximate locales. But that's probably because northern Virginia is like a different state.

The biggest difference comes at breakfast (my favorite meal by far). Anything past about 50 miles south of Washington DC is grits country. I hate grits. Every time my family and friends go out to a restaurant for breakfast in Williamsburg, they're shocked when everyone at the table asks for hash browns instead of grits. Can't stand em.

I'm sure there are more examples, but I can't think of any right now. I haven't had the money to eat out recently
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Matt